Overview

RNA interference (RNAi), in which RNA silences RNA, is the most recent discovery to revolutionize the study of biology. In this book, leaders in the field contribute state-of-the-art, easy to follow methods and bench protocols designed for practical, everyday use of RNAi in biological research. Detailed lists of the necessary equipment and reagents are provided, along with tips on troubleshooting from actual experience of the experts. Divided into two parts, this comprehensive volume covers fundamentals including designs of RNAi, followed by an extensive section covering various applications of RNAi in diverse model organisms and systems, from antiviral and anticancer applications to altering flower color in plants. Cutting edge and clearly written, this book enables a researcher with standard molecular biological training to perform major RNAi-related experiments and contribute to this revolutionary, growing field.