This book throws light on the various methods of preparation and characterization of liposomes. With the advancements in technology since their discovery, newer methods of generating liposomes have been developed. Needless to say, over time liposomes have been modified to a large extent and engineered to suit many of our growing needs. This book throws light on the various methods of preparation and characterization of liposomes. It also discusses the several biochemical and indirect methods that have made it possible to understand the biological and physicochemical mechanisms of liposomes that decide their fate in vivo. In spite of certain limitations, liposomes have proved to be more suitable for a number of unconventional applications. This versatility of liposomes outlined in the book brings out the importance of these nanoparticles in the future applications of nanotechnology besides targeted drug delivery. Overall, this book provides the necessary information about the various aspects of liposomes for beginners.