Rna Abundance Analysis: Methods And Protocols (Methods In Molecular Biology, 2170)

This updated book covers a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches. It includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, organelle RNAs, endosymbiont RNAs, and alternatively spliced RNA variants from various organisms. RNA editing and the computational data processing for genome-wide datasets is also discussed. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, RNA Abundance Analysis: Methods and Protocols, Second Edition provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and other RNA variants, in order to assist researchers in their gene expression studies.

• | Author: Hailing Jin, Isgouhi Kaloshian
• | Publisher: Humana
• | Publication Date: Aug 15, 2020
• | Number of Pages: 244 pages
• | Language: English
• | Binding: Hardcover
• | ISBN-10: 1071607421
• | ISBN-13: 9781071607428