Documenting the exponential growth in cryo-electron microscopy, the resulting advances, new hypotheses, and heightened understanding of the ultrastructure of living and inert matter, this book employes an accessible, step-by-step format to present the most sophisticated methods used in research laboratories today. It describes the basic principles of cryo-fixation, cryo-ultramicrotomy, embedding at low temperatures, and freeze-fracture. It explores applications in electron crystallography, 2D/3D structure analysis, cryo-electron tomography, and techniques for protein localization. Each chapter covers a specific method, including detailed protocols, summary tables, and hundreds of illustrations and diagrams.
- | Author: Annie Cavalier
- | Publisher: CRC Press
- | Publication Date: Oct 02, 2008
- | Number of Pages: 706 pages
- | Binding: Hardback or Cased Book
- | ISBN-10: 0849372275
- | ISBN-13: 9780849372278
