This volume describes breeding methods for the development of biparental and multiparental mapping populations. Chapters detail lab protocols for high-throughput isolation of nucleic acids and metabolites, high performing genotyping approaches, mapping strategies for QTLs, mutation identifications, computational, bioinformatic pipelines, tissue culture-based and transformation methods for androgenesis, ploidy modification, and RNA interference. Additional chapters highlight recent developed genome editing protocols including CRISPR and TALEN methods and methodologies for in-field/in-soil plant phenotyping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Crop Breeding: Genetic Improvement Methods aims to ensure successful results in the further study of this vital field.
- | Author: Pasquale Tripodi
- | Publisher: Humana
- | Publication Date: Dec 04, 2021
- | Number of Pages: 292 pages
- | Language: English
- | Binding: Paperback/Medical
- | ISBN-10: 1071612034
- | ISBN-13: 9781071612033
