Stem Cell Transcriptional Networks: Methods And Protocols (Methods In Molecular Biology, 1150)

Stem Cell Transcriptional Networks: Methods and Protocols collects techniques used to increase our understanding of the underlying transcriptional programs of stem cells that promote self-renewal and differentiation. The volume opens with a section on next-generation sequencing library preparation and data analysis. Continuing with a collection of protocols on visual analysis and interpretation of large-scale interaction networks, this detailed compilation features transcriptional networks in embryonic and adult stem cells, embryo culture and derivation of stem cells, as well as transcriptional programs that promote self-renewal, reprogramming, and transdifferentiation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stem Cell Transcriptional Networks: Methods and Protocols aims to provide a key resource for biologists seeking to interrogate these vital networks.

• | Author: Benjamin L Kidder, Benjamin L. Kidder
• | Publisher: Humana
• | Publication Date: Aug 23, 2016
• | Number of Pages: 296 pages
• | Language: English
• | Binding: Paperback/Science
• | ISBN-10: 1493954067
• | ISBN-13: 9781493954063