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Type 3 Secretion Systems: Methods And Protocols (Methods In Molecular Biology, 1531)
Humana
ISBN13:
9781493966479
$118.37
This volume discusses various basic and advanced methods and protocols that have been proven to be successful among certain bacterial species, or a family of species, in type III secretion systems (T3S system). The chapters in this book cover topics such as: site-directed mutagenesis and its application in studying the interactions of T3S components; use of transcriptional control to increase expression and secretion of heterologous proteins in T3S systems; fractionation techniques to examine effector translocation; detecting immune responses to T3S systems; mouse immunization with purified needle proteins from T3S systems and the characterization of the immune responses to these proteins; and detection of protein interactions in T3S systems using yeast 2-hybrid analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and comprehensive, Type 3 Secretion Systems: Methods and Protocols is a valuable resource for anyone interested in learning about the fascinating and ever-changing T3S systems.
- | Author: Matthew L. Nilles|Danielle Condry, Matthew L. Nilles
- | Publisher: Humana
- | Publication Date: Nov 12, 2016
- | Number of Pages: 234 pages
- | Language: English
- | Binding: Hardcover/SCIENCE
- | ISBN-10: 1493966472
- | ISBN-13: 9781493966479
- Author:
- Matthew L. Nilles|Danielle Condry, Matthew L. Nilles
- Publisher:
- Humana
- Publication Date:
- Nov 12, 2016
- Number of pages:
- 234 pages
- Language:
- English
- Binding:
- Hardcover/SCIENCE
- ISBN-10:
- 1493966472
- ISBN-13:
- 9781493966479