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Phosphoproteomics Analysis Of The Systemin Signaling Pathway
Cuvillier
ISBN13:
9783736970465
$62.40
$60.39
Systemin is a small peptide with important functions in plant wound response signaling. To elucidate systemin perception and signal transduction mechanisms, a phosphoproteomic profiling study was performed to reconstruct a systemin-specific kinase/phosphatase signaling network. Time course analyses revealed early events at the plasma membrane, such as dephosphorylation of H+-ATPase, and the phosphorylation of NADPH-oxidase and Ca2+-ATPase in response to systemin. Later responses included transient phosphorylation of small GTPases and vesicle trafficking proteins, as well as transcription factors. Based on a correlation analysis of systemin-induced phosphorylation profiles, substrate candidates for 44 systemin-responsive kinases and 9 phosphatases were predicted, some of which are involved in a regulatory circuit for the regulation of the plasma membrane H+-ATPase. In this regulatory model, H+-ATPase LHA1 is rapidly de-phosphorylated at its C-terminal regulatory residue T955 by phosphatase PLL5, resulting in the alkalization of the growth medium within two minutes of systemin treatment. LHA1 is re-activated by MAP-Kinase MPK2 later in the systemin response. A valuable resource of proteomic events involved in the systemin signaling cascade is provided with a focus on the prediction of substrates to early systemin-responsive kinases and phosphatases.
- | Author: Fatima Haj Ahmad
- | Publisher: Cuvillier
- | Publication Date: Jul 02, 2019
- | Number of Pages: 190 pages
- | Language: English
- | Binding: Paperback
- | ISBN-10: 3736970463
- | ISBN-13: 9783736970465
- Author:
- Fatima Haj Ahmad
- Publisher:
- Cuvillier
- Publication Date:
- Jul 02, 2019
- Number of pages:
- 190 pages
- Language:
- English
- Binding:
- Paperback
- ISBN-10:
- 3736970463
- ISBN-13:
- 9783736970465