An Ex Vivo 3D Tumor Microenvironment-Mimicry Culture to Study TAM Modulation

Tumor-associated macrophages (TAMs) accumulate in the solid tumor microenvironment (TME) and have been shown to promote tumor growth and dampen antitumor immune responses. TAM-mediated suppression of T-cell antitumor reactivity is considered to be a major obstacle for many immunotherapies, including immune checkpoint blockade and adoptive T/CAR-T-cell therapies. An ex vivo culture system closely mimicking the TME can greatly facilitate the study of cancer immunotherapies. Here, we report the development of a 3D TME-mimicry culture that is comprised of the three major components of a human TME, including human tumor cells, TAMs, and tumor antigen-specific T cells. This TME-mimicry culture can readout the TAM-mediated suppression of T-cell antitumor reactivity, and therefore can be used to study TAM modulation of T-cell-based cancer immunotherapy. As a proof-of-principle, the studies of a PD-1/PD-L1 blockade therapy and a MAO-A blockade therapy were performed and validated.

• | Author: Lili Yang, Yan-Ruide Li
• | Publisher: Eliva Press
• | Publication Date: Sep 22, 2022
• | Number of Pages: 69 pages
• | Language: English
• | Binding: Paperback
• | ISBN-10: 9994982893
• | ISBN-13: 9789994982899